Catalog No: OKCD02319
Size:96WELLS
Price: $650.00
SKU
OKCD02319
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Datasheets/ManualsPrintable datasheet for CD38 ELISA Kit (Rat) (OKCD02319)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)89-96%96-105%97-105%81-91%
EDTA Plasma (n=5)84-102%81-93%83-97%92-102%
Heparin Plasma (n=5)93-101%80-94%87-95%83-95%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 38 (CD38). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 38 (CD38). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 38 (CD38), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 38 (CD38) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)97-104101
EDTA Plasma (n=5)95-104101
Heparin Plasma(n=5)80-9688
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 38 (CD38) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 38 (CD38) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CD38 Microplate96 Wells (12 x 8 Well strips)
CD38 Lyophilized Standard2
100X Biotinylated CD38 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity1.24 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cyclic ADP Ribose Hydrolase (cADPRH). No significant cross-reactivity or interference between Cyclic ADP Ribose Hydrolase (cADPRH) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCd38
Gene Full NameCD38 molecule
Alias Symbols2'-phospho-ADP-ribosyl cyclase;2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase;2'-phospho-cyclic-ADP-ribose transferase;ADPRC 1;ADP-ribosyl cyclase 1;ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1;cADPr hydrolase 1;CD38 antigen (ADP-ribosyl cyclase / cyclic ADP-ribose hydrolase);CD38H;cyclic ADP-ribose hydrolase 1.
NCBI Gene Id25668
Protein NameADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1
Description of TargetSynthesizes the second messagers cyclic ADP-ribose and nicotinate-adenine dinucleotide phosphate, the former a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity.
Uniprot IDQ64244
Protein Accession #NP_037259.1
Nucleotide Accession #NM_013127.1
Protein Size (# AA)303
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