CD36 Antibody (OAEE00832)

Catalog No: OAEE00832

Size:100T

Surface staining of CD36 in human peripheral blood with anti-CD36 (CB38) APC.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: CB38 (NL07)
• Isotype: IgM
• Host: Mouse
• Conjugation: APC
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: living human myeloid cells
• Purification: Purified by size-exclusion chromatography
• Concentration: 10 ul / test
• Specificity: The mouse monoclonal antibody CB38 (NL07) recognizes CD36 (GPIIIb), a 85-113 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes.
  HLDA IV.; WS Code P106
• Formulation: APC
• Storage Buffer: The reagent is provided in Tris buffered saline (TBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
• Reference: Moniuszko M, Kowal K, Rusak M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin Vaccine Immunol. 2006 Jun;13(6):704-7.
  McKenna RW, Washington LT, Aquino DB, Picker LJ, Kroft SH: Immunophenotypic analysis of hematogones (B-lymphocyte precursors) in 662 consecutive bone marrow specimens by 4-color flow cytometry. Blood. 2001 Oct 15;98(8):2498-507.
  Kapinsky M, Torzewski M, Büchler C, Duong CQ, Rothe G, Schmitz G: Enzymatically degraded LDL preferentially binds to CD14(high) CD16(+) monocytes and induces foam cell formation mediated only in part by the class B scavenger-receptor CD36. Arterioscler Thromb Vasc Biol. 2001 Jun;21(6):1004-10.
  Hoffmann PR, deCathelineau AM, Ogden CA, Leverrier Y, Bratton DL, Daleke DL, Ridley AJ, Fadok VA, Henson PM: Phosphatidylserine (PS) induces PS receptor-mediated macropinocytosis and promotes clearance of apoptotic cells. J Cell Biol. 2001 Nov 12;155(4):649-59.
  Matasić R, Dietz AB, Vuk-Pavlović S: Dexamethasone inhibits dendritic cell maturation by redirecting differentiationof a subset of cells. J Leukoc Biol. 1999 Dec;66(6):909-14.
  Fadok VA, Warner ML, Bratton DL, Henson PM: CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3). J Immunol. 1998 Dec 1;161(11):6250-7.
  Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ockenhouse CF, Jamieson GA, Malavasi F: Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgMmonoclonal antibody NL07. Blood. 1993 Dec 15;82(12):3637-47.
  Alessio M, Roggero S, Bussolino F, Saitta M, Malavasi F: Characterization of the murine monoclonal antibody NL07 specific for the human thrombospondin receptor (CD36 molecule). Curr Stud Hematol Blood Transfus. 1991;(58):182-6.
  Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F: Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. Cell Immunol. 1991 Oct 15;137(2):487-500.
  And many other.
  Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  

Target Info

• Gene Symbol: CD36
• Gene Full Name: CD36 molecule
• Alias Symbols: BDPLT10;CD36 antigen (collagen type I receptor, thrombospondin receptor);CD36 molecule (thrombospondin receptor);CHDS7;cluster determinant 36;FAT;fatty acid translocase;glycoprotein IIIb;GP3B;GP4;GPIIIB;GPIV;leukocyte differentiation antigen CD36;PAS IV;PAS-4;PAS-4 protein;PASIV;Platelet collagen receptor;platelet glycoprotein 4;platelet glycoprotein IV;SCARB3;scavenger receptor class B, member 3;Thrombospondin receptor.
• NCBI Gene Id: 948
• Protein Name: Platelet glycoprotein 4
• Description of Target: Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed:18353783, PubMed:21610069). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed:18753675). Involved in oral fat perception and preferences (PubMed:22240721, PubMed:25822988). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellular calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed:20037584). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed:16880211).
• Uniprot ID: P16671
• Protein Accession #: NP_000063.2; NP_001001547.1; NP_001001548.1; NP_001120915.1; NP_001120916.1; NP_001276837.1
• Nucleotide Accession #: NM_000072.3; NM_001001547.2; NM_001001548.2; NM_001127443.1; NM_001127444.1; NM_001289908.1