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Catalog No: OKEH01394
Price: $525.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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CD34 ELISA Kit (Human) : 96 Wells (OKEH01394)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CD34 ELISA Kit (Human) (OKEH01394) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD34 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD34 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD34 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.6% (n = 20)
Mean Inter-assay CV%: < 7.1% (n = 20)
ELISA Kit Component
CD34 Microplate96 Wells (12 x 8 Well strips)
CD34 Lyophilized Standard2
100X Biotinylated CD34 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Hematopoietic progenitor cell antigen CD34
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCD34
Gene Full NameCD34 molecule
Alias SymbolsCD34 antigen;hematopoietic progenitor cell antigen CD34.
NCBI Gene Id947
Protein NameP28906
Description of TargetHematopoietic progenitor cell antigen CD34
Uniprot ID
Protein Accession #NP_001020280.1
Nucleotide Accession #NM_001025109.1
Protein Size (# AA)385
Molecular Weight41 kDa
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