Catalog No: OKEH03363
Size:96 Wells
Price: $625.00
SKU
OKEH03363
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH03363
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CD33 ELISA Kit (Human) (OKEH03363) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD33 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for CD33 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD33 captured in well.
ELISA Kit Range78 - 5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.5% (n = 20)
Mean Inter-assay CV%: <= 11.2% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-CD33 Microplate96 Wells (12 x 8 Well strips)
CD33 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CD33 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.6 pg/mL
SpecificityNatural and recombinant Human Myeloid cell surface antigen CD33
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCD33
Gene Full NameCD33 molecule
Alias Symbolsp67, SIGLEC3, SIGLEC-3
NCBI Gene Id945
Protein Namemyeloid cell surface antigen CD33 isoform 2 precursor
Description of TargetPutative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).
Swissprot IdP20138
Protein Accession #NP_001076087.1
Nucleotide Accession #NM_001082618.1
Protein Size (# AA)364
Molecular Weight40 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express CD33.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express CD33.
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