Size:25 ug
$149.00
Discontinued
SKU
OASA01906
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More Information
Predicted Species Reactivity Human
Product Format Purified IgG - liquid
Clonality Monoclonal
Clone WM53
Isotype IgG1
Host Mouse
Application IHC-AFF, FC, IP
Additional Information Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Histology Positive Control Tissue: Lymphatic tissue
:: Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:: Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Reconstitution and Storage Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Replacement Item This antibody may replace item sc-114798 from Santa Cruz Biotechnology.
Immunogen The immunogen for anti-CD33 antibody: human AML cells
Predicted Homology Based on Immunogen Sequence Human
Datasheets/Manuals Printable datasheet for anti-CD33 antibody - OASA01906
Specificity CD33
Application Info Flow Cytometry:    1/10 - 1/50
Immunohistology - Frozen(1):    1/50 - 1/100
(1) See [*] for special conditions.
Application Data Application #1: Staining of human peripheral blood monocytes with Mouse anti Human CD33
Application #2: Staining of human peripheral blood monocytes with Mouse anti Human CD33:Alexa Fluor 488
Application #3: Staining of human peripheral blood monocytes with Mouse anti Human CD33:Alexa Fluor 647
Application #4: Staining of human peripheral blood monocytes with Mouse anti Human CD33:APC
Application #5: Staining of human peripheral blood monocytes with Mouse anti Human CD33:FITC
Protocol Information Citation: 1: Hernández-Caselles T, Martínez-Esparza M, Pérez-Oliva AB, Quintanilla-Cecconi AM, García-Alonso A, Alvarez-López DM, García-Peñarrubia P. A study of CD33 (SIGLEC-3) antigen expression and function on activated human T and NK cells: two isoforms of CD33 are generated by alternative splicing. J Leukoc Biol. 2006 Jan;79(1):46-58. PubMed PMID: 16380601.
Species: Human
Experiment Name: Immunoprecipitation (IP)
Experiment Background: IP assays using WM53 anti-CD33 mAb were performed to compare lymphoid and myeloid CD33 antigen
Experimental Steps: Cell-surface labeling and IPCell-surface proteins were labeled by biotinylation according to the protocol of the cellular labeling kit used (Boehringer Mannhein, Germany). Briefly, cells were washed twice in phosphate-buffered saline (PBS), pelleted, and resuspended in borate buffer at 107 cells/ml. Biotinylation reagent was added at 5 ug/ml and incubated under agitation at room temperature in the dark. After 15 min of incubation, NH4Cl (50 mM, final concentration) was added to stop the reaction and block free biotin. Then cells were washed twice in cold PBS and prepared for pervanadate stimulation or lysis and IP. Nonstimulated cells were resuspended in lysis buffer [50 mM Tris/ClH, pH 7.5, 150 mM NaCl, 1% Nonidet P-40 (NP-40), 1 mM phenylmethylsulfonyl fluoride (PMSF)] containing 2 ug/ml protease inhibitors (leupeptin, aprotinin, chemostatin, pepstatin). After 20 min on ice, lysates were centrifuged at 12,500 rpm for 15 min, precleared twice with protein A-Sepharose CL-4B beads (Amersham Pharmacia Biotech, Uppsala, Sweden), and immunoprecipitated overnight with protein A-Sepharose CL-4B beads, which had been preincubated previously with anti-CD33 mAb (Clone WM53). Subsequently, immunoprecipitates were washed five times with lysis buffer, resuspended in electrophoresis sample buffer, boiled at 95C for 5 min, and separated by SDS-polyacrylamide gel electrophoresis (PAGE). Finally, isolated immunoprecipitates were blotted onto polyvinylidene difluoride membranes, probed with horseradish peroxidase (HRP)-conjugated streptavidin, and developed using the enhanced chemiluminiscence (ECL) method (Amersham Pharmacia Biotech).
Other Reagents Used: Glutamax-I (L-alanyl-L-glutamine)
Number Of Protocols: 1
Target Reference 1. Favaloro, E.J. et al. (1987) Characterisation of monoclonal antibodies to the human myeloid-specific antigen 'gp67' (CD-33). Dis Markers. 5: 215-225.
2. Favaloro, E.J. et al. (1988) Further characterisation of myeloid antigens ('gp160,95', 'gp150' and 'gp67'): Investigation of epitopic heterogeneity and non-haemopoietic distribution using panels of monoclonal antibodies belonging to CD-11b, CD-13 and CD33. Br. J. Haematol. 69: 163-171.
Gene Symbol CD33
Alias Symbols p67, SIGLEC3, FLJ00391, SIGLEC-3, CD33
NCBI Gene Id 945
Protein Name Myeloid cell surface antigen CD33
Description of Target MOUSE ANTI HUMAN CD33
Swissprot Id P20138
Protein Accession # NP_001763.3
Protein Size (# AA) 364
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express CD33.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express CD33.
  1. What is the species homology for "CD33 Antibody (OASA01906)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".

  2. How long will it take to receive "CD33 Antibody (OASA01906)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "CD33 Antibody (OASA01906)" provided in?

    This item is provided in "Purified IgG - liquid ".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "CD33 Antibody (OASA01906)"?

    This target may also be called "p67, SIGLEC3, FLJ00391, SIGLEC-3, CD33" in publications.

  5. What is the shipping cost for "CD33 Antibody (OASA01906)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "CD33 Antibody (OASA01906)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "CD33 Antibody (OASA01906)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "CD33 Antibody (OASA01906)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "CD33"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CD33"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CD33"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CD33"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CD33"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CD33"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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