CD33 Antibody (OAAB07161)

Catalog No: OAAB07161

Size:400ul

CD33 Antibody(Cat. #OAAB07161) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CD33 antibody detected the CD33 protein (arrow).

Fluorescent image of A549 cell stained with CD33 Antibody(Cat#OAAB07161/Lot# SM120504AC).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CD33 primary antibody (1:25, 1 h at 37C. For secondary antibody, Alexa Fluor488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37C. Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).CD33 immunoreactivity is localized to Membrane significantly.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Clone: 574CT16.4.4
• Isotype: IgM
• Host: Mouse
• Application: WB, IF
• Additional Information: Function: Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro)
  Cellular Location: Cell membrane; Single-pass type I membrane protein
  Tissue Location: Monocytic/myeloid lineage cells.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CD33 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 337-364 amino acids from human CD33.
• Purification: This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IF: 1:10~50
  WB: 1:100~500
• Intended Use: CD33 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Target Info

• Gene Symbol: CD33
• Alias Symbols: p67, SIGLEC3, SIGLEC-3
• NCBI Gene Id: 945
• Protein Name: Myeloid cell surface antigen CD33
• Description of Target: Mouse Monoclonal Antibody (Mab)
• Uniprot ID: P20138
• Protein Accession #: NP_001763.3
• Molecular Weight: 39825 Da