Catalog No: OKWB00239
Size:96T
Price: $450.00
SKU
OKWB00239
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Datasheets/ManualsPrintable datasheet for CD302 ELISA Kit (Mouse) (OKWB00239)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)85-101%87-105%86-99%92-103%
EDTA Plasma (n=5)90-100%83-99%83-101%89-97%
Heparin Plasma (n=5)81-100%80-91%81-96%80-100%
ELISA Kit PrincipleAviva Systems Biology Cd302 ELISA Kit (Mouse) (OKWB00239) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd302 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd302 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd302 captured in the well.
ELISA Kit Range46.875-3000pg/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)91-10096
EDTA Plasma (n=5)86-9892
Heparin Plasma (n=5)89-9491
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Cd302 Microplate96 Wells (12 x 8 Well strips)
Cd302 Lyophilized Standard2 x 3 ng
100X Cd302 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity28.1 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolCd302
Gene Full NameCD302 antigen
Alias Symbols1110055L24Rik;AI159627;CD302 antigen;C-type lectin domain family 13 member A;type I transmembrane C-type lectin receptor DCL-1.
NCBI Gene Id66205
Protein NameCD302 antigen
Description of TargetPotential multifunctional C-type lectin receptor that may play roles in endocytosis and phagocytosis as well as in cell adhesion and migration.
Uniprot IDQ9DCG2
Protein Accession #NP_001277589
Nucleotide Accession #NM_001290660
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