CD300LF ELISA Kit (Human) (OKBB01353)

Catalog No: OKBB01353

Size:96 wells

CD300LF ELISA Kit (Human) (OKBB01353) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse CD300F ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD300F has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: T20-S156) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD300F is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substmousee TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	60	272	844	67	385	987
  Standard deviation	3.72	14.42	39.67	4.09	28.49	50.34
  CV(%)	6.2	5.3	4.7	6.1	7.4	5.1

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human CD300LF Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human CD300LF Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human CD300LF Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	60	53	52	60	56	3.76	6.7%
  Sample 2	272	252	233	247	251	13.98	5.5%
  Sample 3	844	800	822	904	842	38.76	4.6%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
  O.D.	0.024	0.117	0.203	0.382	0.766	1.049	1.787	2.421

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human CD300F
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: T20-S156
• Description: For quantitative detection of human CD300F in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CD300LF
• Gene Full Name: CD300 molecule like family member f
• Alias Symbols: CMRF35-like molecule 1; CLM-1; CD300 antigen-like family member F; Immune receptor expressed on myeloid cells 1; IREM-1; Immunoglobulin superfamily member 13; IgSF13; NK inhibitory receptor; CD300f; CD300LF; CD300F; CLM1; IGSF13; IREM1; NKIR; UNQ3105/PRO10111
• NCBI Gene Id: 146722
• Protein Name: CMRF35-like molecule 1
• Description of Target: CMRF35-like molecule 1 is a protein that in humans is encoded by the CD300LF gene. It is mapped to 17q25.1. This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants.
• Uniprot ID: Q8TDQ1
• Protein Accession #: NP_001276011.1
• Nucleotide Accession #: NM_001289082.1