CD300LB ELISA Kit (Mouse) (OKEH03622)

Catalog No: OKEH03622

Size:96T

Mouse CD300LB ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CD300LB ELISA Kit (Mouse) (OKEH03622) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd300lb has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cd300lb is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd300lb captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.9% (n = 20)
  Mean Inter-assay CV%: <= 8.2% (n = 20)
• ELISA Kit Component:
  • Cd300lb Microplate: 96 Wells (12 x 8 Well strips)
  • Cd300lb Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Cd300lb Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 41 pg/mL
• Specificity: Natural and recombinant Mouse CMRF35-like molecule 7
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cd300lb
• Gene Full Name: CD300 molecule like family member B
• Alias Symbols: CD300 antigen like family member B;CD300 antigen-like family member B;Clm;CLM-;Clm7;CLM-7;CMRF35-like molecule 7;CMRF-35-like molecule-7;Gm253;immune receptor expressed on myeloid cells 3;IREM-3;leukocyte mono-Ig-like receptor 5;LM;LMIR5.
• NCBI Gene Id: 217304
• Protein Name: Q3U497
• Description of Target: CMRF35-like molecule 7
• Uniprot ID: https://www.uniprot.org/uniprot/Q3U497
• Protein Accession #: NP_954691.2
• Nucleotide Accession #: NM_199221.2
• Protein Size (# AA): 209
• Molecular Weight: 24 kDa