CD274 Antibody : PerCP-Cy5.5 (OATA00507)

Catalog No: OATA00507

Human PBMC were stimulated for 3 days with PHA and stained with 5 uL (1 ug) PerCP-Cyanine5.5 Anti-Human CD274 (OATA00507) (solid line) or 1 ug PerCP-Cyanine5.5 Mouse IgG2b isotype control (dashed line).

Product Info

• Tested Species Reactivity: Human
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: 29E.2A3
• Isotype: Mouse IgG2b, kappa
• Host: Mouse
• Conjugation: PERCP: Peridinin Chlorophyll Protein Complex, CY5.5: Cyanine 5.5
• Application: FC
  Excitation Laser: Blue (488 nm)
  Emmision: 676 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: PD-L1 (B7-H1)
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.

Target Info

• Gene Symbol: CD274
• Gene Full Name: CD274 molecule
• Alias Symbols: B7-H, B7H1, PDL1, PD-L1, hPD-L1, PDCD1L1, PDCD1LG1
• NCBI Gene Id: 29126
• Protein Name: programmed cell death 1 ligand 1
• Description of Target: This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.