CD274 Antibody : APC (OATA00069)

Catalog No: OATA00069

C57Bl/6 splenocytes were stained with 0.25 ug APC Anti-Mouse CD274 (OATA00069) (solid line) or 0.25 ug APC Rat IgG2b isotype control (dashed line).

Product Info

• Tested Species Reactivity: Mouse
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: 10F.9G2
• Isotype: Rat IgG2b, kappa
• Host: Rat
• Conjugation: APC: Allophycocyanin
• Application: FC
  Excitation Laser: Red (633-640 nm)
  Emmision: 660 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD274 (PD-L1, B7-H1)
• Application Info: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
• Reference:

  Chen A, Ahlen G, Brenndorfer ED, Brass A, Holmstrom F, Chen M, Soderholm J, Milich DR, Frelin L, and Sallberg M. 2011. J. Immunol. 186:5107-5118. (in vivo depletion)

  Zhang L, Gajewski TF, and Kline J. 2009. Blood. 114:1545-1552. (in vivo blocking)

  Rivas MN, Weatherly K, Hazzan M, Vokaer B, Dremier S, Gaudray F, Goldman M, Salmon I, and Braun MY. 2009. 183:4284-4291. (in vitro blocking)

  Brooks DG, Ha S-J, Elsaesser H, Sharpe AH, Freeman GJ, and Oldstone MBA. 2008. Proc. Natl. Acad. Sci. 105:20428-20433. (in vivo blocking)

  Koehn BH, Ford ML, Ferrer IR, Borom K, Gangappa S, Kirk AD, and Larsen CP. 2008. J. Immunol. 181:5313-5322. (in vivo blocking)

  Chen A, Ahlen G, Brenndorfer ED, Brass A, Holmstrom F, Chen M, Soderholm J, Milich DR, Frelin L, and Sallberg M. 2011. J. Immunol. 186:5107-5118. (in vivo depletion)

  Zhang L, Gajewski TF, and Kline J. 2009. Blood. 114:1545-1552. (in vivo blocking)

  Rivas MN, Weatherly K, Hazzan M, Vokaer B, Dremier S, Gaudray F, Goldman M, Salmon I, and Braun MY. 2009. 183:4284-4291. (in vitro blocking)

  Brooks DG, Ha S-J, Elsaesser H, Sharpe AH, Freeman GJ, and Oldstone MBA. 2008. Proc. Natl. Acad. Sci. 105:20428-20433. (in vivo blocking)

  Koehn BH, Ford ML, Ferrer IR, Borom K, Gangappa S, Kirk AD, and Larsen CP. 2008. J. Immunol. 181:5313-5322. (in vivo blocking)

Target Info

• Gene Symbol: CD274
• Gene Full Name: CD274 antigen
• Alias Symbols: PD-, B7h1, Pdl1, Pdcd1l, Pdcd1l1, Pdcd1lg1, A530045L16Rik
• NCBI Gene Id: 60533
• Protein Name: programmed cell death 1 ligand 1
• Description of Target: The protein encoded by this gene is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Mice deficient for this gene display a variety of phenotypes including decreased allogeneic fetal survival rates and severe experimental autoimmune encephalomyelitis.