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CD27 Antibody - PE Conjugated (OASA01736)

250 ul
$179.00
In Stock
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Gene Symbol:
CD27
NCBI Gene Id:
939
Protein Name:
CD27 antigen
Swissprot Id:
P26842
Protein Accession #:
NP_001233.1
Alias Symbols:
T14, S152, Tp55, TNFRSF7, MGC20393, CD27
Replacement Item:
This antibody may replace item sc-1740 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI HUMAN CD27:RPE
Protein Size (# AA):
260
Host:
Mouse
Clonality:
Monoclonal
Application:
FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD27.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD27.
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Reconstitution and Storage:
Reconstitution: Reconstitute in 0.25 ml disilled water
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-CD27 antibody - OASA01736
Specificity:
CD27
Clone:
LT27
Conjugation:
RPE
Isotype:
IgG2a
Application Info:
Flow Cytometry:    Neat
Application Data:
Application #1: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27
Application #2: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27: Alexa Fluor 647
Application #3: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27:FITC
Application #4: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27:RPE
Additional Information:
Fusion Partners: Spleen cells from immunised mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein G
Preservative Stabilisers: 0.09% - Sodium Azide
1% - Bovine Serum Albumin
:::
Approx Protein Conc: IgG concentration 0.1 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes. Vet Immunol Immunopathol. 119: 115-22.
2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49: 97-110.
3. Ravoet, A.M. et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. Oxford University Press pp 408-411.

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