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CD27 Antibody (OASA01732)

1 mg
$1,045.00
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Gene Symbol:
CD27
NCBI Gene Id:
939
Protein Name:
CD27 antigen
Swissprot Id:
P26842
Protein Accession #:
NP_001233.1
Alias Symbols:
T14, S152, Tp55, TNFRSF7, MGC20393, CD27
Replacement Item:
This antibody may replace item sc-1740 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI HUMAN CD27:Azide Free
Protein Size (# AA):
260
Host:
Mouse
Clonality:
Monoclonal
Application:
FC, IP
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD27.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD27.
Predicted Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for anti-CD27 antibody - OASA01732
Specificity:
CD27
Clone:
LT27
Conjugation:
Azide Free
Isotype:
IgG2a
Application Info:
Flow Cytometry:    1/100
Application Data:
Application #1: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27
Application #2: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27: Alexa Fluor 647
Application #3: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27:FITC
Application #4: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27:RPE
Additional Information:
Fusion Partners: Spleen cells from immunised mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.
:::
Preparation: Purified IgG prepared by ion exchange chromatography
Preservative Stabilisers: None present
:::
Approx Protein Conc: IgG concentration 1.0 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Target Reference:
1. Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes. Vet Immunol Immunopathol. 119: 115-22.
2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49: 97-110.
3. Ravoet, A.M. et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. Oxford University Press pp 408-411.

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