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CD27 Antibody (OASA01731)

  • Catalog#: OASA01731
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  • This product is Genway GWB-C9B10F
Predicted Species Reactivity:
Human
Product Format:
Purified IgG - liquid
Clonality:
Monoclonal
Clone:
LT27
Isotype:
IgG2a
Host:
Mouse
Application:
FC, IP
Additional Information:
Fusion Partners: Spleen cells from immunised mice were fused with cells of the P3. X 63-Ag8-653 mouse myeloma cell line.
:::
Preparation: Purified IgG prepared by ion exchange chromatography
Preservative Stabilisers: 0.09% - Sodium Azide
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Approx Protein Conc: IgG concentration 40 ug/ml
Buffer Solutions: Phosphate buffered saline
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Symbol:
CD27
NCBI Gene Id:
939
Protein Name:
CD27 antigen
Swissprot Id:
P26842
Protein Accession #:
NP_001233.1
Alias Symbols:
T14, S152, Tp55, TNFRSF7, MGC20393, CD27
Replacement Item:
This antibody may replace item sc-1740 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI HUMAN CD27
Protein Size (# AA):
260
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD27.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD27.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Printable datasheet for anti-CD27 antibody - OASA01731
Specificity:
CD27
Application Info:
Flow Cytometry:    1/50 - 1/100
Application Data:
Application #1: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27
Application #2: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27: Alexa Fluor 647
Application #3: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27:FITC
Application #4: Staining of human peripheral blood lymphocytes with Mouse anti Human CD27:RPE
Target Reference:
1. Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes. Vet Immunol Immunopathol. 119: 115-22.
2. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim. 49: 97-110.
3. Ravoet, A.M. et al. (1989) CD25 mAb: epitopes recognised, effect on lymphocyte activation, mediation of ADCC in Leucocyte Typing IV: White Cell Differentiation Antigens Eds Knapp, W. et al. Oxford University Press pp 408-411.

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