CD25/IL-2sR alpha ELISA Kit (Mouse) (OKBB00458)

Catalog No: OKBB00458

Size:96 Wells

Mouse CD25/IL-2sR alpha ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse CD25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD25 has been precoated onto 96-well plates. Standards (NS0,E22-K236) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD25 amount of sample captured in plate.
• ELISA Kit Range: 78 pg/ml - 5,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	564	1244	2860	758	1654	3242
  SD	32.7	75.9	157.3	54.6	134	217
  CV%	5.8	6.1	5.5	7.2	8.1	6.7

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse IL-2sR alpha Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse IL-2sR alpha Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse IL-2sR alpha Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <5 pg/ml
• Specificity: Natural and recombinant mouse CD25
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: E22-K236
• Description: For quantitative detection of mouse CD25 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: IL2RA
• Gene Full Name: interleukin 2 receptor, alpha
• Alias Symbols: CD122, CD122 antigen, CD132, CD132 antigen, CD25, CD25 antigen, Cytokine receptor common gamma chain, Gamma C, High affinity IL 2 receptor subunit beta, IDDM10, IL 2 RA, IL 2 receptor, IL 2 receptor alpha subunit, IL 2R gamma chain, IL2 RA, IL2R, IL2RA, IL2RB, IL2RG, IMD4, interleukin 2 receptor alpha, Interleukin 2 receptor alpha chain, interleukin 2 receptor beta, Interleukin 2 receptor beta chain, interleukin 2 receptor gamma, Interleukin 2 receptor gamma (severe combined immunodeficiency), Interleukin 2 receptor gamma chain, p55, p64, P70 75, p75, SCIDX, SCIDX1, TAC antigen, TCGFR, XSCID
• NCBI Gene Id: 16184
• Protein Name: Interleukin-2 receptor subunit alpha
• Description of Target: CD25 is the alpha chain of the IL-2 receptor. T cellsIt is a type I transmembrane protein present on activated , activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.
• Uniprot ID: P01590
• Protein Accession #: NP_032393.3
• Nucleotide Accession #: NM_008367.3