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Datasheets/Manuals | Printable datasheet for OATA00013 |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Chimpanzee |
Product Format | Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
Clonality | Monoclonal |
Clone | OKT3 |
Isotype | Mouse IgG2a, kappa |
Host | Mouse |
Conjugation | APC: Allophycocyanin |
Application | FC |
:: | Excitation Laser: Red (633-640 nm) |
:: | Emmision: 660 nm |
Reconstitution and Storage | 2-8C protected from light |
Specificity | CD3 |
Application Info | This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells. |
Reference | Haga-Friedman A, Horovitz-Fried M, and Cohen CJ. Jun 2012. J. Immunol. 188:5538-5546. (in vitro activation) Bikker A, Moret FM, Kruize AA, Bijlsma JWJ, Lafeber FPJG, and van Roon JAG. Jun 2012. Rheumatology 51:996-1005 (in vitro activation) Bagnara D, Kaufman MS, Calissano C, et al. 2011. Blood. 117: 5463-5472. (in vivo depletion) Nguyen V, Cao L, Lin JT, Hung N, Ritz A, Yu K, Jianu R, Ulin SP, Raphael BJ, Laidlaw DH, Brossay L, and Salomon AR. 2009. Mol. Cell. Proeomics. 8: 2418-2431. (in vitro activation) Bibollet-Ruche F, McKinney BA, Duverger A, Wagner FH, Ansari AA, and Kutsch O. 2008. J. Virol. 82(20): 10271-10278. (in vitro activation - Chimpanzee) Roura-Mir C, Catalfamo M, Cheng TY, Marqusee E, Besra GS, Jaraquemada D, and Moody DB. 2005. J. Immunol. 174:3773-3780 (Immunohistochemistry - Acetone fixed frozen sections) Sato Y, Mukai K, Watanabe S, Goto M, and Shimosato Y. 1986. Am. J. Pathol. 125(3):431-435. (Immunohistochemistry - Paraffin embedded sections) |
Gene Symbol | CD247, CD3D, CD3E, CD3G |
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Gene Full Name | CD247 molecule |
Alias Symbols | T3D, IMD19, CD3-DELTA |
NCBI Gene Id | 915; 916; 917; 919 |
Protein Name | T-cell surface glycoprotein CD3 zeta chain |
Description of Target | The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. |
Uniprot ID | P20963, A8MVP6, P07766, P09693 |
Protein Accession # | NP_000725.1 |
Nucleotide Accession # | NM_000734.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CD247/CD3D/CD3E/CD3G Antibody : APC (OATA00013)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Chimpanzee".
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How long will it take to receive "CD247/CD3D/CD3E/CD3G Antibody : APC (OATA00013)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "CD247/CD3D/CD3E/CD3G Antibody : APC (OATA00013)" provided in?
This item is provided in "Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CD247/CD3D/CD3E/CD3G Antibody : APC (OATA00013)"?
This target may also be called "T3D, IMD19, CD3-DELTA" in publications.
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What is the shipping cost for "CD247/CD3D/CD3E/CD3G Antibody : APC (OATA00013)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CD247/CD3D/CD3E/CD3G Antibody : APC (OATA00013)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CD247/CD3D/CD3E/CD3G Antibody : APC (OATA00013)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CD247/CD3D/CD3E/CD3G Antibody : APC (OATA00013)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CD247, CD3D, CD3E, CD3G"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CD247, CD3D, CD3E, CD3G"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CD247, CD3D, CD3E, CD3G"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CD247, CD3D, CD3E, CD3G"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CD247, CD3D, CD3E, CD3G"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CD247, CD3D, CD3E, CD3G"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.