Catalog No: OKEH01549
Size:96T
Price: $600.00
SKU
OKEH01549
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CD24 ELISA Kit (Human) : 96 Wells (OKEH01549)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CD24 ELISA Kit (Human) (OKEH01549) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD24 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD24 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD24 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
CD24 Microplate96 Wells (12 x 8 Well strips)
CD24 Lyophilized Standard2
100X Biotinylated CD24 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Human Signal transducer CD24
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCD24
Gene Full NameCD24 molecule
Alias SymbolsCD24 antigen (small cell lung carcinoma cluster 4 antigen);CD24A;signal transducer CD24;Small cell lung carcinoma cluster 4 antigen.
NCBI Gene Id100133941
Protein NameP25063
Description of TargetSignal transducer CD24
Uniprot IDhttps://www.uniprot.org/uniprot/P25063
Protein Accession #NP_001278666.1
Nucleotide Accession #NM_001291737.1
Protein Size (# AA)80
Molecular Weight8 kDa
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