Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any help please email info@avivasysbio.com

Catalog No: OKCD00754 (Formerly GWB-KBBQR7)
Size:96WELLS
Price: $650.00
SKU
OKCD00754
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for CD226 ELISA Kit (Human) (OKCD00754)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)78-102%96-105%85-98%94-101%
EDTA plasma(n=5)86-93%90-97%86-93%86-103%
heparin plasma(n=5)86-101%86-99%95-105%85-93%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 226 (CD226). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 226 (CD226). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 226 (CD226), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 226 (CD226) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)83-9187
EDTA plasma(n=5)83-9086
heparin plasma(n=5)86-9490
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 226 (CD226) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 226 (CD226) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CD226 Microplate96 Wells (12 x 8 Well Strips)
CD226 Lyophilized Standard2 x 2 ng
100X Biotinylated CD226 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 12.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 226 (CD226). No significant cross-reactivity or interference between Cluster Of Differentiation 226 (CD226) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCD226
Gene Full NameCD226 molecule
Alias Symbolsadhesion glycoprotein;CD226 antigen;DNAM1;DNAM-1;DNAX accessory molecule 1;DNAX accessory molecule-1;platelet and T cell activation antigen 1;PTA1;T lineage-specific activation antigen 1 antigen;TLiSA1.
NCBI Gene Id10666
Protein NameCD226 antigen
Description of TargetInvolved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell (PubMed:8673704). Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding (PubMed:26755705).
Uniprot IDQ15762
Protein Accession #NP_001290547
Nucleotide Accession #NM_001303618.1
Protein Size (# AA)336
Write Your Own Review
You're reviewing:CD226 ELISA Kit (Human) (OKCD00754)
Your Rating
We found other products you might like!