CD22 ELISA Kit (Human) (OKCD07174)

Catalog No: OKCD07174

Size:96WELLS

CD22 ELISA Kit (Human) (OKCD07174) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialic Acid Binding Ig Like Lectin 2 (CD22). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sialic Acid Binding Ig Like Lectin 2 (CD22). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sialic Acid Binding Ig Like Lectin 2 (CD22), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Sialic Acid Binding Ig Like Lectin 2 (CD22) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sialic Acid Binding Ig Like Lectin 2 (CD22) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sialic Acid Binding Ig Like Lectin 2 (CD22) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CD22 Microplate: 96 Wells (12 x 8 Well strips)
  • CD22 Lyophilized Standard: 2
  • 100X CD22 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids.
• Sensitivity: < 1.14ng/mL

Target Info

• Gene Symbol: CD22
• Gene Full Name: CD22 molecule
• Alias Symbols: B-cell receptor CD22;BL-CAM;B-lymphocyte cell adhesion molecule;CD22 antigen;sialic acid-binding Ig-like lectin 2;SIGLEC2;SIGLEC-2;T-cell surface antigen Leu-14.
• NCBI Gene Id: 933
• Protein Name: B-cell receptor CD22
• Description of Target: Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.
• Uniprot ID: P20273
• Protein Accession #: NP_001172028.1
• Nucleotide Accession #: NM_001185099.1