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Catalog No: OKWB00151
Size:96T
Price: $450.00
SKU
OKWB00151
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Datasheets/ManualsPrintable datasheet for CD21 ELISA Kit (Human) (OKWB00151)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)91-100%87-100%93-104%85-100%
EDTA Plasma (n=5)89-95%82-90%83-101%83-100%
Heparin Plasma (n=5)84-100%83-100%80-93%80-94%
ELISA Kit PrincipleAviva Systems Biology CD21 ELISA Kit (Human) (OKWB00151) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD21 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD21 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD21 captured in the well.
ELISA Kit Range0.313-20ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)87-10195
EDTA Plasma (n=5)85-10491
Heparin Plasma (n=5)89-10094
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
CD21 Microplate96 Wells (12 x 8 Well strips)
CD21 Lyophilized Standard2 x 20 ng
100X CD21 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.188 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolCR2
Gene Full Namecomplement C3d receptor 2
Alias SymbolsC3DR;CD21;Complement C3d receptor;complement component (3d/Epstein Barr virus) receptor 2;complement component 3d receptor 2;complement receptor type 2;CR;CVID7;EBV receptor;Epstein-Barr virus receptor;SLEB9.
NCBI Gene Id1380
Protein NameComplement receptor type 2
Description of TargetReceptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRNPU (PubMed:7753047). Participates in B lymphocytes activation (PubMed:7753047).
Uniprot IDP20023
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