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Catalog No: OKEH04724
Size:96T
Price: $600.00
SKU
OKEH04724
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH04724
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology CD209 ELISA Kit (Human) (OKEH04724) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD209 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD209 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD209 captured in well.
ELISA Kit Range78-5000pg/ml
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
CD209 Microplate96 Wells (12 x 8 Well Strips)
CD209 Standard2
Sample Diluent1 x 20 mL
CD209 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity40.9 pg/mL
SpecificityNatural and recombinant Human CD209 antigen
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCD209
Gene Full NameCD209 molecule
Alias SymbolsCD209 antigen;CDSIGN;CLEC4L;C-type lectin domain family 4 member L;DC-SIGN;DC-SIGN1;dendritic cell-specific ICAM-3-grabbing non-integrin 1;dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin;dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin;hDC-SIGN;HIV gpl20-binding protein.
NCBI Gene Id30835
Protein NameQ9NNX6
Description of TargetCD209 antigen
Uniprot IDhttps://www.uniprot.org/uniprot/Q9NNX6
Protein Accession #NP_001138365.1
Nucleotide Accession #NM_001144893.1
Protein Size (# AA)404
Molecular Weight46 kDa
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