CD200 ELISA Kit (Human) (OKBB00486)

Catalog No: OKBB00486

Size:96 Wells

Human CD200 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human CD200 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CD200 has been precoated onto 96-well plates. Standards(NS0, Q31-G232) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD200 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CD200 amount of sample captured in plate.
• ELISA Kit Range: 31.2 pg/ml - 2,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	120	635	1149	130	623	1208
  SD	6.12	34.29	70.09	8.06	35.51	90.6
  CV%	5.1	5.4	6.1	6.2	5.7	7.5

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human CD200 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human CD200 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human CD200 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human CD200
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q31-G232
• Description: For quantitative detection of human CD200 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CD200
• Gene Full Name: CD200 molecule
• Alias Symbols: antigen identified by monoclonal antibody MRC OX-2, CD_antigen=CD200, CD200 antigen, MOX1, MOX2, MRC OX-2 antigen, My033, OX-2 membrane glycoprotein
• NCBI Gene Id: 4345
• Protein Name: OX-2 membrane glycoprotein
• Description of Target: OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
• Uniprot ID: P41217
• Protein Accession #: NP_001004196.2
• Nucleotide Accession #: NM_001004196.2