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Catalog No: OKEH04730
Size:96T
Price: $525.00
SKU
OKEH04730
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Cd19 ELISA Kit (Mouse) (OKEH04730) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd19 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd19 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cd19 captured in well.
ELISA Kit Range1.25-80ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Cd19 Microplate96 Wells (12 x 8 Well Strips)
Cd19 Standard2
Sample Diluent1 x 20 mL
Cd19 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.633 ng/mL
SpecificityNatural and recombinant Mouse B-lymphocyte antigen CD19
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCd19
Gene Full NameCD19 antigen
Alias SymbolsAW495831;B-lymphocyte antigen CD19;differentiation antigen CD19.
NCBI Gene Id12478
Protein NameP25918
Description of TargetB-lymphocyte antigen CD19
Uniprot IDhttps://www.uniprot.org/uniprot/P25918
Protein Accession #NP_033974.2
Nucleotide Accession #NM_009844.2
Protein Size (# AA)547
Molecular Weight60 kDa
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