Catalog No: OKDB00015
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OKDB00015
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CD178 ELISpot Assay Kit (Human) (OKDB00015)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Duration< 4 Hours
ELISA Kit PrincipleAviva's Human CD178 ELISpot Kit is based on standard sandwich ELISA methodology. A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.
ELISA Kit ComponentReagents sufficient for 96 Wells:
1. Pre-coated 96 well PVDF bottomed plates (1x96 Wells)
2. Biotinylated detection antibody
3. Streptavidin-Alkaline Phosphatase conjugate
4. Bovine Serum Albumin
5. Ready to use BCIP/NBT substrate buffer
Reconstitution and Storage2 to 8C, Do not use past expiration date.
Sample TypeCell Suspensions
Assay InfoAssay Methodology: Quantitative Sandwich ELISpot Immunoassay
Gene SymbolCD178
Gene Full NameFas ligand
Alias SymbolsAPTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, TNLG1A, APT1LG1
NCBI Gene Id356
Protein Nametumor necrosis factor ligand superfamily member 6
Description of TargetThis gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described.
Uniprot IDP48023
Protein Accession #NP_000630.1
Nucleotide Accession #NM_000639.2
Protein Size (# AA)281
Molecular Weight31 kDa
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