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Catalog No: OKBB00596
Size:96 Wells
Price: $876.00
SKU
OKBB00596
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's mouse CD169 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD169 has been precoated onto 96-well plates. Standards(NS0, T20-R1639) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD169 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CD169 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)1.644.856.882.545.317.05
SD0.060.2040.3160.1190.270.437
CV%3.74.24.64.75.16.2
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Mouse CD169 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Mouse CD169 Standard2 x 10 ng/tube
100X Biotinylated Anti-Mouse CD169 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant mouse CD169
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: T20-R1639
DescriptionFor quantitative detection of mouse CD169 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolSIGLEC1
Gene Full Namesialic acid binding Ig-like lectin 1, sialoadhesin
Alias SymbolsCD169, CD169 antigen, dJ1009E24.1, DKFZp667F058, FLJ00051, FLJ00055, FLJ00073, FLJ00411, FLJ32150, Sialic acid binding Ig like lectin 1, Sialic acid binding immunoglobulin like lectin 1, Sialic acid-binding Ig-like lectin 1, Sialoadhesin, Siglec 1, Siglec-1, Siglec1, SN, SN_HUMAN
NCBI Gene Id20612
Protein NameSialoadhesin
Description of TargetSIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily (IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
Uniprot IDQ62230
Protein Accession #NP_035556.3
Nucleotide Accession #NM_011426.3
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