CD163 ELISA Kit (Rat) (OKCD00177)

Catalog No: OKCD00177 (Formerly GWB-KBBHF3)

Size:96WELLS

Rat Cd163 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	90-98%	81-96%	94-104%	86-99%
  EDTA plasma(n=5)	95-102%	78-103%	96-104%	99-105%
  heparin plasma(n=5)	99-105%	78-99%	89-96%	88-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation (CD163). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation (CD163). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation (CD163), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation (CD163) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	89-97	93
  EDTA plasma(n=5)	82-94	86
  heparin plasma(n=5)	95-103	101

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation (CD163) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation (CD163) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Cd163 Microplate: 96 Wells (12 x 8 Well Strips)
  • Cd163 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated Cd163 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 6.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation (CD163). No significant cross-reactivity or interference between Cluster Of Differentiation (CD163) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Cd163
• Gene Full Name: CD163 molecule
• Alias Symbols: CD163 antigen;cluster of differentiation 163;scavenger receptor cysteine-rich type 1 protein M130.
• NCBI Gene Id: 312701
• Protein Name: CD163 antigen (Predicted)
• Description of Target: CD163 antigen (Predicted)
• Uniprot ID: D3Z9U2
• Protein Accession #: NP_001101357
• Nucleotide Accession #: NM_001107887.1
• Protein Size (# AA): 1120