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- $55: Antibody & Protein in US
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| Datasheets/Manuals | Printable datasheet for CD163 Antibody (OASA11557) |
|---|
| Predicted Species Reactivity | Rat |
|---|---|
| Product Format | Liquid. Phosphate buffered saline |
| Clonality | Monoclonal |
| Clone | ED2 |
| Isotype | IgG1 |
| Host | Mouse |
| Application | Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot |
| Additional Information | Endotoxin Level: < 0.01 EU/ug Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0-Ag 14 mouse myeloma cell line. Histology Positive Control Tissue: Liver Carrier Free: Yes |
| Reconstitution and Storage | -20°C |
| Immunogen | Rat Spleen cell homogenate. |
| Purification | Protein A Purified |
| Concentration | 1 mg/ml |
| Specificity | Mouse anti Rat CD163, clone ED2 recognises the rat ED2 cell surface glycoprotein. |
| Application Info | FC: Use 10 ul of the suggested working dilution to label 106 cells in 100 ul. IHC-P: This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Optimal dilutions should be determined by the end user. |
| Gene Symbol | CD163 |
|---|---|
| Gene Full Name | Scavenger receptor cysteine-rich type 1 protein M130 |
| Alias Symbols | MOUSE ANTI RAT CD163: Low Endotoxin, ED2 |
| NCBI Gene Id | 312701 |
| Protein Name | Scavenger receptor cysteine-rich type 1 protein M130 |
| Description of Target | A 175 kDa molecule also known as rat CD163, a member of the group B scavenger receptor cysteine-rich (SRCR) family and an erythroblast adhesion receptor. Mouse anti rat CD163, clone ED2 was shown to detect approximately 50% of peritoneal macrophages, a subset of splenic macrophages, and most tissue macrophages. However, no staining was observed in monocytes or alveolar macrophages. In freshly isolated bone marrow, expression of CD163 was limited to mature macrophages only. Clone ED2 may be used in immunohistology using antigen retrieval, and has also been described reacting with paraffin-embedded material following PLP fixation (Periodate-lysine-paraformaldehyde). |
| Uniprot ID | D3Z9U2 |
| Molecular Weight | 175 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CD163 Antibody (OASA11557)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Rat".
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How long will it take to receive "CD163 Antibody (OASA11557)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "CD163 Antibody (OASA11557)" provided in?
This item is provided in "Liquid. Phosphate buffered saline".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CD163 Antibody (OASA11557)"?
This target may also be called "MOUSE ANTI RAT CD163: Low Endotoxin, ED2" in publications.
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What is the shipping cost for "CD163 Antibody (OASA11557)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CD163 Antibody (OASA11557)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CD163 Antibody (OASA11557)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "175 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CD163 Antibody (OASA11557)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CD163"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CD163"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CD163"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CD163"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CD163"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CD163"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
