CD163 Antibody (OABF01014)

Catalog No: OABF01014

Size:100UL

L1 rat brain, L2 mouse uterus lysates probed (OABF01014) at 1:200 in 4C. Followed by conjugation to secondary antibody at 1:3000 90min in 37C. Predicted and observed band size: 130kDa.

Formalin-fixed and paraffin-embedded rat colon labeled with Rabbit Anti-CD163/M130 Polyclonal Antibody, Unconjugated ( OABF01014) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat colon tissue labeled with Anti-CD163/M130 Polyclonal Antibody, Unconjugated (OABF01014) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded Human testis tissue labeled with unconjugated Anti-CD163/M130 Polyclonal Antibody, unconjugated (OABF01014) at 1:100 for 40 minutes at 37C followed by labeling Donkey Anti-Rabbit, Cy3 conjugated 1:300, 60 minutes at 37C. DAPI nuclear stain employed. Image shows membrane staining of testicular macrophages in the interstitial compartment of the testis, while cells in the seminiferous tubules are negative.

Product Info

• Predicted Species Reactivity: Dog|Horse|Human|Mouse|Other|Porcine|Rat
• Product Format: Liquid. 0.01M TBS (pH 7.4) 1% BSA, 50% glycerol and 0.09% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Western blot
  Subcellular Location: Extracellular.
• Reconstitution and Storage: -20°C
• Immunogen: KLH conjugated synthetic peptide derived from human CD163/M130
• Purification: Protein A Purified
• Concentration: 1 ug/ul
• Intended Use: For research use only. Not intended for diagnostic or therapeutic use.
• Application Info: WB: 1:500~2000
  ELISA: 1:5000~10000
  FC: 1:100
  IHC-P: 1:100~500
  IHC-F: 1:100~500
  Fluorescent-IHC-P: 1:100~500
  Fluorescent-IHC-F: 1:100~500
  Fluorescent-ICC: 1:100~500
  ICC: 1:100~500

Target Info

• Gene Symbol: CD163
• Gene Full Name: CD163 molecule
• Alias Symbols: hemoglobin scavenger receptor;M130;macrophage-associated antigen;MM130;SCARI1;scavenger receptor cysteine-rich type 1 protein M130.
• NCBI Gene Id: 9332
• Protein Name: Scavenger receptor cysteine-rich type 1 protein M130
• Description of Target: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
• Uniprot ID: Q86VB7