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Now Offering Over 102,157 Antibodies & 44,722 Antigens!

CD163 antibody - N-terminal region (OAAB04323)

400ul
$289.00
In Stock
Gene Symbol:
CD163
NCBI Gene Id:
9332
Protein Name:
Scavenger receptor cysteine-rich type 1 protein M130
Swissprot Id:
Q86VB7
Protein Accession #:
NP_004235.4
Alias Symbols:
Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, CD_antigen=CD163, Soluble CD163, sCD163, M130, CD163
Replacement Item:
This antibody may replace item sc-18794 from Santa Cruz Biotechnology.
Description of Target:
CD163 is an acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. The protein may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. It binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. And it exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. It also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.
Molecular Weight:
125451 Da
Host:
Rabbit
Clonality:
Polyclonal
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application:
WB, IHC-P
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD163.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD163.
Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Human
Product Format:
Liquid; PBS with 0.09% (W/V) sodium azide.
Reconstitution and Storage:
Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Approximately 0.5mg/ml. Actual concentration varies with each lot.
Datasheets/Manuals:
Printable datasheet for anti-CD163 antibody - OAAB04323
Sample Type Confirmation:

CD163 is strongly supported by BioGPS gene expression data to be expressed in K562

Specificity:
This CD163 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 245-274 amino acids from the N-terminal region of human CD163.
Isotype:
Ig
Application Info:
The suggested dilutions are:
Western blot~ 1:1000
IHC-P~ 1:10~50
Additional Information:
Function Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.

Cellular Location Soluble CD163: Secreted.

Tissue Location Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.

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