Catalog No: OKCD01195 (Formerly GWB-KBBCP4)
Size:96WELLS
Price: $650.00
SKU
OKCD01195
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Datasheets/ManualsPrintable datasheet for CD160 ELISA Kit (Human) (OKCD01195)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)96-104%98-105%86-93%80-95%
EDTA plasma(n=5)78-90%95-102%82-104%84-99%
heparin plasma(n=5)95-104%78-95%85-102%80-92%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cluster Of Differentiation 160 (CD160). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cluster Of Differentiation 160 (CD160). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cluster Of Differentiation 160 (CD160), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cluster Of Differentiation 160 (CD160) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)95-103101
EDTA plasma(n=5)98-105101
heparin plasma(n=5)96-10599
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 160 (CD160) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 160 (CD160) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CD160 Microplate96 Wells (12 x 8 Well Strips)
CD160 Lyophilized Standard2 x 5 ng
100X Biotinylated CD160 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, cell lysatesand other biological fluids.
Sensitivity< 30 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 160 (CD160). No significant cross-reactivity or interference between Cluster Of Differentiation 160 (CD160) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCD160
Gene Full NameCD160 molecule
Alias SymbolsBY55;CD160 antigen;CD160-delta Ig;natural killer cell receptor BY55;natural killer cell receptor, immunoglobulin superfamily member;NK1;NK28.
NCBI Gene Id11126
Protein NameCD160 antigen
Description of TargetReceptor showing broad specificity for both classical and non-classical MHC class I molecules.
Uniprot IDO95971
Protein Accession #NP_008984
Nucleotide Accession #NM_007053.3
Protein Size (# AA)181
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