CD151 Antibody (OASA01305)

Catalog No: OASA01305 (Formerly GWB-A3DFF6)

Size:200UG

Staining of human peripheral blood platelets with Mouse anti Human CD151

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: 11G5a
• Isotype: IgG1
• Host: Mouse
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen|Immunohistochemistry-Paraffin|Immunoprecipitation|Western blot
• Additional Information: Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line.
  Histology Positive Control Tissue: Tonsil/spleen.
  Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
  Preservative Stabilisers: 0.09% - Sodium Azide
  Approx Protein Conc: IgG concentration 1.0 mg/ml
• Reconstitution and Storage: -20°C
• Purification: Protein G Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: CD151
• Application Info: Clone 11G5a recognises human CD151 under non-reducing conditions.
• Application Data: Application #1: Staining of human peripheral blood platelets with Mouse anti Human CD151
• Protocol Information: Citation: 1: Karamatic Crew V, Burton N, Kagan A, Green CA, Levene C, Flinter F, Brady RL, Daniels G, Anstee DJ. CD151, the first member of the tetraspanin (TM4)superfamily detected on erythrocytes, is essential for the correct assembly ofhuman basement membranes in kidney and skin. Blood. 2004 Oct 15;104(8):2217-23.Epub 2004 Jul 20. PubMed PMID: 15265795.
  Species: Human
  Experiment Name: Flow cytometry
  Experiment Background: 1. Vanja et al. conclude that CD151 is essential for the proper assembly of the glomerular and tubular basement membrane in kidney, has functional significance in the skin, is probably a component of the inner ear, and could play a role in erythropoiesis.2. Flow cytometric studies of erythroid precursors (proerythroblasts) cultured from CD34+ cells.3. Expression of MER2 (CD151) on lymphoblastoid cells. 4. Tests with CD61 on platelets are included as a control.
  Experimental Steps: Cultured erythroid progenitor cells, platelets, EBV-transformed lymphocytes, and erythrocytes were analyzed by flow cytometry after labeling with a variety of antibodies: mouse monoclonal anti-CD151 (clone 11G5a; , Kidlington, Oxford, United Kingdom), anti-MER2 (2F7), anti-GPA (glycophorin A) (BRIC256), pooled antibodies to the Lutheran glycoprotein (BRIC221, BRIC224), anti-Rh polypeptide (BRIC69) (all IBGRL, Bristol, United Kingdom), anti-β1 (TS2/16), anti-5 (SNAK52), and anti-CD61 (PM6/13; ) were used as required. An eluate of human anti-M, prepared by adsorption and elution from antigen-positive cells (Elu-Kit II; Gamma Biologicals, Houston, TX), was used in the dual-labeling studies. For single labeling, cells were incubated with primary antibody at room temperature for 30 minutes and washed once. Bound antibody was detected by adding R phycoerythrin (RPE)–conjugated F(ab’)2 anti–mouse globulin (Dako) and incubating at 4C in the dark for 30 minutes. Cells were washed once, and the geometric mean (FL2) was recorded for each test. Cells were dual labeled by incubating the cells consecutively with monoclonal anti-CD151 or anti-MER2 and with an eluate of human anti-M for 60 minutes at room temperature. Cells were washed once, and RPE-conjugated F(ab’)2 anti–mouse globulin (Dako) and fluorescein isothiocyanate (FITC)–conjugated F(ab’)2 fragment anti–human globulin (Dako) were added. After incubation at 4C in the dark the cells were washed once, and FL1 versus FL2 fluorescence was recorded. Flow cytometry was performed on a FacsCalibur flow cytometer (Becton Dickinson, Oxford, United Kingdom) and analyzed with CellQuest software. Isotype controls (Dako) were tested in parallel to ascertain background fluorescence as required; for dual-labeling compensation was set by using single-labeled positive cells prior to acquisition of data.
  Other Reagents Used: Tri-sodium citrate, 2-mercaptoethanol, and L-glutamine
  Number Of Protocols: 1
• Reference: 1. Yauch, R.L. et al. (1998) Highly stoichiometric, stable, and specific association of integrin alpha 3 beta 1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration. Mol. Cell. Biol. 9: 2751-2765.
  2. Crew, V.K. et al. (2004) CD151, the first member of the tetraspanin (TM4) superfamily detected on erythrocytes, is essential for the correct assembly of human basement membranes in kidney and skin. Blood 104:2217-2223.
  3. Zheng, Z. and Liu, Z. (2006) CD151 gene delivery activates PI3K/Akt pathway and promotes neovascularization

Target Info

• Gene Symbol: CD151
• Alias Symbols: GP27, MER2, RAPH, SFA1, PETA-3, TSPAN24
• NCBI Gene Id: 977
• Protein Name: CD151 antigen
• Description of Target: MOUSE ANTI HUMAN CD151
  
• Uniprot ID: P48509
• Protein Accession #: NP_001034579.1
• Protein Size (# AA): 253