CD14 Protein (OPPA01423)

Catalog No: OPPA01423 (Formerly GWB-F05245)

Size:2UG

Product Info

• Predicted Species Reactivity: Mouse
• Product Format: CD14 was lyophilized from a concentrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: Chinese Hamster Ovarian Cells (CHO)
• Application: ELISA, NT, WB
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Mouse CD14 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Product Introduction: CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. CD14 has been shown to bind apoptotic cells.
  Product Description: The CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR). The recombinant mouse CD-14 was purified by His-tag and controled by SDS page.The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.
• Reconstitution and Storage: Lyophilized Mouse CD14 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Mouse CD14 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Application Info: Characterization: On SDS-PAGE commassie blue stained gel, the 95% purified recombinant protein shows a band at 50 kDa.

Target Info

• Gene Symbol: mCD14
• Alias Symbols: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, CD14 Mouse, CD14 Mouse Recombinant
• NCBI Gene Id: 12475
• Protein Name: Monocyte differentiation antigen CD14
• Description of Target: Recombinant Mouse CD14
• Uniprot ID: P10810
• Protein Accession #: NP_033971.1
• Protein Size (# AA): Recombinant