CD14 ELISA Kit (Rat) (OKCA01002)

Catalog No: OKCA01002

Size:96 Wells

Rat CD14 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean n=4 (%)	Range (%)
  Neat	94	86-100
  1:2	99	90-103
  1:4	89	83-92
  1:8	95	84-98

• ELISA Kit Principle: Aviva Systems Biology CD14 ELISA Kit (Rat) (OKCA01002) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CD14 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CD14 captured in well.
• ELISA Kit Range: 28 ng/mL-1,800 ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	102	94-108
  EDTA Plasma (n=4)	96	89-101

• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level CD14 were tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level CD14 were tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <8%
  Inter-Assay CV: <10%
• ELISA Kit Component:
  • CD14 Microplate: 96 Wells (12 x 8 Well strips)
  • CD14 Lyophilized Standard: 2
  • 100X Biotinylated CD14 Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 7 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CD14
• Gene Full Name: Monocyte differentiation antigen CD14
• NCBI Gene Id: 100008983
• Protein Name: Monocyte differentiation antigen CD14
• Description of Target: Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) . Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL-.
• Uniprot ID: Q28680
• Protein Accession #: NP_001075664.1
• Nucleotide Accession #: NM_001082195.2