CD14 ELISA Kit (Human) (OKBB00782)

Catalog No: OKBB00782

Size:96 wells

CD14 ELISA Kit (Human) (OKBB00782) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human CD14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD14 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO, Immunogen sequence: T20-C352) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	310	1057	4116	312	1164	4732
  Standard deviation	13.95	49.7	209.92	18.1	75.66	350.2
  CV(%)	4.5	4.7	5.1	5.8	6.5	7.4

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human CD14 Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human CD14 Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human CD14 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	310	311	305	362	322	23.20	7.2%
  Sample 2	1057	1075	989	1027	1037	32.58	3.1%
  Sample 3	4116	3778	3962	4374	4057	218.42	5.3%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
  O.D.	0.079	0.137	0.191	0.328	0.567	0.973	1.612	2.313

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <50 pg/ml
• Specificity: Natural and recombinant human CD14
• Immunogen: Expression system for standard: CHO; Immunogen sequence: T20-C352
• Description: For quantitative detection of human CD14 in cell culture supernatants, serum and plasma(heparin, EDTA).

Target Info

• Gene Symbol: CD14
• Gene Full Name: CD14 molecule
• Alias Symbols: Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein; CD14
• NCBI Gene Id: 929
• Protein Name: Monocyte differentiation antigen CD14
• Description of Target: CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein(mCD14) and a monocyte or liver-derived soluble serum protein(sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor(along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide(LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein(LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.
• Uniprot ID: P08571
• Protein Accession #: NP_000582.1
• Nucleotide Accession #: NM_000591.3