CD14 ELISA Kit (Bovine) (OKEH07451)

Catalog No: OKEH07451

Size:96T

Bovine CD14 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CD14 ELISA Kit (Bovine) (OKEH07451) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CD14 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CD14 captured in well.
• ELISA Kit Range: 7.8-500ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=6.9% (n = 20)
  Mean Inter-assay CV%: <=7.1% (n = 20)
• ELISA Kit Component:
  • CD14 Microplate: 96 Wells (12 x 8 Well strips)
  • CD14 Lyophilized Standard: 2
  • 100X Biotinylated CD14 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).
  Subcellular Location: Cell membrane Lipid-anchor GPI-anchor Secreted Membrane raft Golgi apparatus Soluble, secreted forms seem to exist. They may arise by cleavage of the GPI anchor.
  Unigene: Bt.4285
  String: 9913.ENSBTAP00000020009
  KEGG: bta:281048
  Pfam: PF13516
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 2.3ng/mL
• Specificity: Natural and recombinant Bovine Monocyte differentiation antigen CD14
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CD14
• Gene Full Name: CD14 molecule
• Alias Symbols: CD14 antigen;monocyte differentiation antigen CD14;myeloid cell-specific leucine-rich glycoprotein.
• NCBI Gene Id: 281048
• Protein Name: Q95122
• Description of Target: Monocyte differentiation antigen CD14
• Uniprot ID: https://www.uniprot.org/uniprot/Q95122
• Protein Accession #: NP_776433.1
• Nucleotide Accession #: NM_174008.1