CD14 antibody

Catalog No: OAEE00081

Size:100UG

Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18).
Lane 1: whole cell lysate HEK 293 transfected with empty vector
Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA
Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA

Surface staining of human peripheral blood leukocytes using anti-human CD14 (clone MEM-18) FITC.

Product Info

• Predicted Species Reactivity: Human|Non-human Primate
• Product Format: Phosphate buffered saline (PBS), pH 7.4
• Clonality: Monoclonal
• Clone: MEM-18
• Isotype: IgG1
• Host: Mouse
• Application: ELISA|FA|FC|IP|WB
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.
  HLDA III; WS Code M 253
  HLDA IV; WS Code M 314
  HLDA V; WS Code M MA087
  HLDA VI; WS Code M MA95
• Formulation: Low endotoxin
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 0.01 EU/ug of the protein, as determined by the LAL test.

Target Info

• Reference: Bazil V, Horejsi V, Baudys M, Kristofova H, Strominger JL, Kostka W, Hilgert I: Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen. Eur J Immunol. 1986 Dec;16(12):1583-9.
  Bazil V, Baudys M, Hilgert I, Stefanova I, Low MG, Zbrozek J, Horejsi V: Structural relationship between the soluble and membrane-bound forms of human monocyte surface glycoprotein CD14. Mol Immunol. 1989 Jul;26(7):657-62.
  Iwaki D, Nishitani C, Mitsuzawa H, Hyakushima N, Sano H, Kuroki Y: The CD14 region spanning amino acids 57-64 is critical for interaction with the extracellular Toll-like receptor 2 domain. Biochem Biophys Res Commun. 2005 Mar 4;328(1):173-6.
  Sing A, Rost D, Tvardovskaia N, Roggenkamp A, Wiedemann A, Kirschning CJ, Aepfelbacher M, Heesemann J: Yersinia V-antigen exploits toll-like receptor 2 and CD14 for interleukin 10-mediated immunosuppression. J Exp Med. 2002 Oct 21;196(8):1017-24.
  Funda DP, Tucková L, Farré MA, Iwase T, Moro I, Tlaskalová-Hogenová H: CD14 is expressed and released as soluble CD14 by human intestinal epithelial cells in vitro: lipopolysaccharide activation of epithelial cells revisited. Infect Immun. 2001 Jun;69(6):3772-81.
  Stöckl J, Majdic O, Fischer G, Maurer D, Knapp W: Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL. J Immunol. 2001 Sep 1;167(5):2724-33.
  Weiss TS, Lichtenauer M, Kirchner S, Stock P, Aurich H, Christ B, Brockhoff G, Kunz-Schughart LA, Jauch KW, Schlitt HJ, Thasler WE. Hepatic progenitor cells from adult human livers for cell transplantation. Gut. 2008 Aug;57(8):1129-38.
  Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
  Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR: CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts. Int Immunol. 2007 Nov;19(11):1271-9.
  Leukocyte Typing III., McMichael A.J. et al. (Eds.), Oxford University Press (1987).
  Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
  Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989).
  Lodrup Carlsen KC, Granum B: Soluble CD14: role in atopic disease and recurrent infections, including otitismedia. Curr Allergy Asthma Rep. 2007 Nov;7(6):436-43.
  Fernández-Real JM, Broch M, Richart C, Vendrell J, López-Bermejo A, Ricart W: CD14 monocyte receptor, involved in the inflammatory cascade, and insulinsensitivity. J Clin Endocrinol Metab. 2003 Apr;88(4):1780-4.
  Asai Y, Makimura Y, Kawabata A, Ogawa T: Soluble CD14 Discriminates Slight Structural Differences between Lipid As That Lead to Distinct Host Cell Activation. J Immunol. 2007 Dec 1;179(11):7674-83.
  Juan TS, Hailman E, Kelley MJ, Wright SD, Lichenstein HS: Identification of a domain in soluble CD14 essential for lipopolysaccharide (LPS) signaling but not LPS binding. J Biol Chem. 1995 Jul 21;270(29):17237-42.
• Gene Symbol: CD14
• Gene Full Name: CD14 molecule
• Alias Symbols: monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein.
• NCBI Gene Id: 929
• Protein Name: Monocyte differentiation antigen CD14
• Description of Target: Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-) (PubMed:23880187).
• Uniprot ID: P08571
• Protein Accession #: NP_000582.1; NP_001035110.1; NP_001167575.1; NP_001167576.1
• Nucleotide Accession #: NM_000591.3; NM_001040021.2; NM_001174104.1; NM_001174105.1