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Catalog No: OKBB00544
Size:96 Wells
Price: $583.00
SKU
OKBB00544
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human CD13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CD13 has been precoated onto 96-well plates. Standards(NS0, K69-K967) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CD13 amount of sample captured in plate.
ELISA Kit Range312 pg/ml - 20,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)2.986.5412.733.276.8313.76
SD0.1580.360.7510.190.430.922
CV%5.35.55.95.86.36.7
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human CD13 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human CD13 Standard2 x 20 ng/tube
100X Biotinylated Anti-Human CD13 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human CD13
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: K69-K967
DescriptionFor quantitative detection of Human CD13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolANPEP
Gene Full Namealanyl (membrane) aminopeptidase
Alias SymbolsAlanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Alanyl membrane aminopeptidase, Aminopeptidase M, Aminopeptidase N, AMPN_HUMAN, ANPEP, AP M, AP N, AP-M, AP-N, APN, CD 13, CD13, CD13 antigen, gp150, hAPN, Lap 1, Lap1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, p150, PEPN
NCBI Gene Id290
Protein NameAminopeptidase N
Description of TargetAlanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.
Uniprot IDP15144
Protein Accession #NP_001141.2
Nucleotide Accession #NM_001150.2
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