CCT8L1P ELISA Kit (Human) (OKEH01970)

Catalog No: OKEH01970

Size:96T

Human CCT8L1P ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CCT8L1P ELISA Kit (Human) (OKEH01970) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CCT8L1P antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CCT8L1P and incubated. The CCT8L1P found in the sample or standards competes with the biotinylated CCT8L1P for limited binding sites on the immobilized anti-human CCT8L1P antibody. Excess unbound biotinylated CCT8L1P and sample or standard CCT8L1P is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CCT8L1P captured in the well and inversely proportional to the amount of CCT8L1P which was contained in the sample or standard.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.3% (n = 20)
  Mean Inter-assay CV%: < 9.2% (n = 20)
• ELISA Kit Component:
  • Anti-CCT8L1P Microplate: 96 Wells (12 x 8 Well strips)
  • CCT8L1P Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X CCT8L1P-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 38 pg/mL
• Specificity: Natural and recombinant Human Putative T-complex protein 1 subunit theta-like 1
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: CCT8L1P
• Gene Full Name: chaperonin containing TCP1 subunit 8 like 1, pseudogene
• Alias Symbols: CCT8L1
• NCBI Gene Id: 155100
• Protein Name: A6NM43
• Description of Target: Putative T-complex protein 1 subunit theta-like 1
• Uniprot ID: https://www.uniprot.org/uniprot/A6NM43
• Protein Size (# AA): 557
• Molecular Weight: 59 kDa