Catalog No: OKEH03441
Size:96 Wells
Price: $525.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CCR5 ELISA Kit (Mouse) (OKEH03441) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccr5 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Ccr5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ccr5 captured in well.
ELISA Kit Range0.156 - 10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 94%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 8.6% (n = 20)
Mean Inter-assay CV%: <= 10.7% (n = 20)
ELISA Kit Component
Anti-Ccr5 Microplate96 Wells (12 x 8 Well strips)
Ccr5 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Ccr5 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.086 ng/mL
SpecificityNatural and recombinant Mouse C-C chemokine receptor type 5
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCCR5
Gene Full Namechemokine (C-C motif) receptor 5
Alias SymbolsAM4-7, CD195, Cmkbr, Cmkbr5
NCBI Gene Id12774
Protein NameC-C chemokine receptor type 5(C-C CKR-5;CC-CKR-5;CCR-5)
Description of TargetReceptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation.
Uniprot IDP51682
Protein Accession #NP_034047.2
Nucleotide Accession #NM_009917.5
Protein Size (# AA)354
Molecular Weight41 kDa
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