CCR3 Antibody - middle region (OAAB05403)

Catalog No: OAAB05403

Size:400UL

CCR3 is a receptor for C-C type chemokines. It belongs to family 1 of the G protein-coupled receptors. This receptor binds and responds to a variety of chemokines, including eotaxin (CCL11), eotaxin-3 (CCL26), MCP-3 (CCL7), MCP-4 (CCL13), and RANTES (CCL5). It is highly expressed in eosinophils and basophils, and is also detected in TH1 and TH2 cells, as well as in airway epithelial cells. This receptor may contribute to the accumulation and activation of eosinophils and other inflammatory cells in the allergic airway. It is also known to be an entry co-receptor for HIV-1.

Western blot analysis of CCR3 Antibody (Center) in Jurkat cell lysates (35ug/lane). CCR3 (arrow) was detected using the purified Pab.

CCR3 Antibody (Center) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Primate
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: Flow cytometry, Western blot
• Additional Information: Cellular Location: Cell membrane; Multi-pass membrane protein.
  Tissue Location: In eosinophils as well as trace amounts in neutrophils and monocytes.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This CCR3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-318 amino acids from the Central region of human CCR3.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 0.5 mg/ml
• Application Info: WB: 1:1000
  FC: 1:10~50
• Other Applications Image 1 Data: CCR3 Antibody (Center) flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
• Publications:

  Simvastatin Inhibits IL-5-Induced Chemotaxis and CCR3 Expression of HL-60-Derived and Human Primary Eosinophils. PLoS ONE. 11, e0157186 (2016). 27275740

Target Info

• Gene Symbol: CCR3
• Gene Full Name: C-C motif chemokine receptor 3
• Alias Symbols: b-chemokine receptor;C C CKR3;CC chemokine receptor 3;C-C chemokine receptor type 3;C-C CKR-3;CC-CKR-3;CCR-3;CD193;chemokine (C-C motif) receptor 3;CKR 3;CKR3;CMKBR3;eosinophil CC chemokine receptor 3;eosinophil eotaxin receptor.
• NCBI Gene Id: 1232
• Protein Name: NP_001828.1, NP_847898.1, NP_847899.1, P51677, Q9BDS8
• Description of Target: C-C chemokine receptor type 3, C-C chemokine receptor type 3 isoform 1 [Homo sapiens], C-C chemokine receptor type 3 isoform 2 [Homo sapiens]
• Uniprot ID: https://www.uniprot.org/uniprot/?query=P51677+OR+Q9BDS8
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001828.1,NP_847898.1,NP_847899.1
• Molecular Weight: 41044