Catalog No: OKEI00400
Size:96 Wells
Price: $625.00
SKU
OKEI00400
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- $55: Antibody & Protein in US
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Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Mouse | ||||||||||||||||||||||
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ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Duration | ~ 3 Hours | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Ccp110 ELISA Kit (Mouse) (OKEI00400) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ccp110 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ccp110 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Ccp110 | ||||||||||||||||||||||
ELISA Kit Range | 0.156 - 10 ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Ccp110. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Ccp110 using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sample Type | Serum, Plasma, Biological Fluids | ||||||||||||||||||||||
Sensitivity | 0.094 ng/mL | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | CCP110 |
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Alias Symbols | CP11, CP110, cep110, AA415922, AI427129, AW557948, 6330503K22Rik |
NCBI Gene Id | 101565 |
Protein Name | Centriolar coiled-coil protein of 110 kDa |
Description of Target | Necessary for centrosome duplication at different stages of procentriole formation. Acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation . Also involved in promoting ciliogenesis. May play a role in the assembly of the mother centriole subdistal appendages (SDA) thereby effecting the fusion of recycling endosomes to basal bodies during cilia formation . Required for correct spindle formation and has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CETN2. |
Uniprot ID | Q7TSH4 |
Protein Accession # | NP_892040 |
Nucleotide Accession # | NM_182995 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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