CCND3 Antibody (Phospho-Thr283) (OAAF00073)

Catalog No: OAAF00073 (Formerly GWB-ASB097)

Size:100 ug

Western blot analysis of extracts from K562 cells treated with UV 5', using Cyclin D3 (Phospho-Thr283) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Cyclin D3 (Phospho-Thr283) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Cyclin D3 around the phosphorylation site of Thr283.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Specificity: Cyclin D3 (Phospho-Thr283) antibody detects endogenous levels of Cyclin D3 only when phosphorylated at threonine 283.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1000; IHC: 1:50~1:100; ELISA: 1:1000
• Reference: Guo-Li Wang, Mol. Cell. Biol., Apr 2006; 26: 2570 - 2582.
  Feng Dong, Mol. Biol. Cell, Aug 1998; 9: 2081.
  Tapan Kumar Bagui, Mol. Cell. Biol., Dec 2000; 20: 8748 - 8757.
  David A. Sarruf, Mol. Cell. Biol., Nov 2005; 25: 9985 - 9995.

Target Info

• Gene Symbol: CCND3
• Gene Full Name: cyclin D3
• Alias Symbols: D3-type cyclin;G1/S-specific cyclin-D3.
• NCBI Gene Id: 896
• Protein Name: G1/S-specific cyclin-D3
• Description of Target: Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
• Uniprot ID: P30281
• Protein Accession #: NP_001129489.1
• Molecular Weight: 32 kDa