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CCND1 Antibody - middle region (ARP30163_P050)

100 ul
$289.00
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Conjugation Options

ARP30163_P050-FITC Conjugated

ARP30163_P050-HRP Conjugated

ARP30163_P050-Biotin Conjugated

Tested Species Reactivity:
Human
Predicted Species Reactivity:
Cow, Dog, Horse, Human, Pig, Rabbit, Rat, Yeast, Zebrafish
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Clonality:
Polyclonal
Host:
Rabbit
Application:
WB
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Gene Symbol:
CCND1
Official Gene Full Name:
Cyclin D1
NCBI Gene Id:
595
Protein Name:
G1/S-specific cyclin-D1
Swissprot Id:
P24385
Protein Accession #:
NP_444284
Nucleotide Accession #:
NM_053056
Alias Symbols:
BCL1, D11S287E, PRAD1, U21B31
Replacement Item:
This antibody may replace item sc-166288 from Santa Cruz Biotechnology.
Description of Target:
CCND1 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Protein Size (# AA):
295
Molecular Weight:
34kDa
Purification:
Affinity Purified
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CCND1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CCND1.
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human CCND1
Predicted Homology Based on Immunogen Sequence:
Cow: 92%; Dog: 92%; Horse: 92%; Human: 100%; Pig: 91%; Rabbit: 83%; Rat: 85%; Yeast: 83%; Zebrafish: 79%
Complete computational species homology data:
Anti-CCND1 (ARP30163_P050)
Peptide Sequence:
Synthetic peptide located within the following region: ACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
CDKN1B; CDKN1A; CDK4; RELA; FBXO31; UBC; FBXO4; CAMK1; TP73; RB1; CDK2; HSPA8; POLR1B; MCM10; ORC4; LPL; BRINP1; CDK6; RABEP1; CARD11; CUL1; RBL2; UBTF; CCND1; ATF2; CDKN2A; FBXW8; BTRC; PPP1CC; PPP1CB; PSMA6; ITLN1; MCM7; MCM6; MCM3; MCM2; ELAVL1; PCNA;
Blocking Peptide:
For anti-CCND1 (ARP30163_P050) antibody is Catalog # AAP30163 (Previous Catalog # AAPS08607)
Datasheets/Manuals:
Printable datasheet for anti-CCND1 (ARP30163_P050) antibody
Target Reference:
Kibel,A.S., (2008) Prostate 68 (11), 1179-1186

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02/01/2017 15:23
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Product Review: CCND1 antibody - middle region (ARP30163_P050) in PKO + Doxor lysate using Western Blot

Product Page for CCND1 antibody - middle region (ARP30163_P050)

Researcher: Tobias Wagner, Institute for Biochemistry and Biophysics
Application: Western Blotting
Species + Tissue/Cell type: Lane 1: 30ug PKO lysate Lane 2: 30ug PKO + Doxor lysate Primary antibody dilution: 1:1000
Secondary antibody: Goat anti rabbit-HRP
Secondary antibody dilution: 1:5000

Questionnaire:  
How do Aviva's reagents play a role in your experimental goals? Cell cycle regulation; possible target gene in my experiments
How would you rate this antibody on a scale from 1-5 (5=best) nad why? 4
Would you use this antibody in future experiment? Yes
Have you used another antibody which has worked in your application? Yes
Do you believe the information about the reagent on Aviva's website is correct? Yes
If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not? Maybe
How did you store the antibody after re-suspension?  4C
Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel): RKO colon cancer cells, untreated and treated w/ 1 uM doxorubicin 6h
How many different experimental trials were conducted using the antibody sample? 2
How was this sample prepared? Lysis in NETN buffer
Primary antibody dilution and incubation time: 1:1000 in 2% milk in PBS-T, o/n 4C
Secondary antibody used and dilution and incubation time: 1:5000 in 2% milk in PBS-T, 1 h RT
Please include your detailed WB Procedure/Protocol here: Cell lysate (30 ug) from RKO cells; untreated or treated with Doxorubicin (DoxoR) 1uM 24h
SDS-PAGE: 10/13.5% 2 layer discontinuous gel; marker Pierce PageRuler Plus prestained
BioRad MiniProtean 3; gel run: 20mA/gel 1 h; transfer: 20mA/gel o/n

block: 5% dry milk in PBS-T 1h RT
1° Ab: 1:1000 in 2% dry milk in PBS-T (+ NaN3 0.01%); 4C o/n
wash: 3x 10min PBS-T
2° Ab: gt anti-rb (Pierce) 1:5000 in 2% dry milk in PBS-T; 1h RT
wash: 3x 10min PBS-T
detection: ECL SuperSignal West Pico Chemiluminescent Substrate (Pierce)
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