Catalog No: OKEH07219
Size:96T
Price: $800.00
SKU
OKEH07219
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Bos taurus, Bovine | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology CCN2 ELISA Kit (Bovine) (OKEH07219) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCN2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCN2 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 83% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=3.2% (n = 20) Mean Inter-assay CV%: <=12.3% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity). | ||||||||||||||||||||||
:: | Subcellular Location: Secreted Extracellular space Extracellular matrix Secreted | ||||||||||||||||||||||
:: | Unigene: Bt.5240 SMR: O18739 String: 9913.ENSBTAP00000008357 | ||||||||||||||||||||||
:: | KEGG: bta:281103 Pfam: PF00007 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.15ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Bovine CCN family member 2 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | CCN2 |
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Gene Full Name | cellular communication network factor 2 |
Alias Symbols | CCN family member 2;connective tissue growth factor;CTGF. |
NCBI Gene Id | 281103 |
Protein Name | O18739 |
Description of Target | Connective tissue growth factor |
Uniprot ID | https://www.uniprot.org/uniprot/O18739 |
Protein Accession # | NP_776455.1 |
Nucleotide Accession # | NM_174030.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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