CCL9 ELISA Kit (Mouse) (OKBB00549)

Catalog No: OKBB00549

Size:96 Wells

Mouse CCL9 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's mouse CCL9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL9 has been precoated onto 96-well plates. Standards(E.coli, Q22-Q122) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse CCL9 amount of sample captured in plate.
• ELISA Kit Range: 7.8 pg/ml - 500 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	34	78	294	35	82	302
  SD	1.19	3.67	14.99	1.68	4.59	19.03
  CV%	3.5	4.7	5.1	4.8	5.6	6.3

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Mouse CCL9 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Mouse CCL9 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Mouse CCL9 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <1 pg/ml
• Specificity: Natural and recombinant mouse CCL9
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: Q22-Q122
• Description: For quantitative detection of mouse CCL9 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CCL9
• Gene Full Name: chemokine (C-C motif) ligand 9
• Alias Symbols: CCF18, CCL9, Chemokine CC motif ligand 9, Macrophage Inflammatory Protein 1 gamma, Macrophage inflammatory protein-related protein 2, MIP-1 gamma, mMIP1g, SCYA10, SCYA9, Small inducible cytokine A9
• NCBI Gene Id: 20308
• Protein Name: C-C motif chemokine 9
• Description of Target: Chemokine (C-C motif) ligand 9 (CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium (FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1 (the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.
• Uniprot ID: P51670
• Protein Accession #: NP_035468.1
• Nucleotide Accession #: NM_011338.2