CCL8/MCP-2 ELISA Kit (Human) (OKBB00367)

Catalog No: OKBB00367

Size:96 Wells

Human CCL8/MCP-2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human CCL8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from goat specific for CCL8 has been precoated onto 96-well plates. Standards(E.coli, Q24-P99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CCL8 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	144	388	607	158	406	627
  SD	7.5	25.61	24.28	9.8	28.83	30.1
  CV%	5.2	6.6	4	6.2	7.1	4.8

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human MCP-2 Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human MCP-2 Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human MCP-2 Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants serum and plasma (heparin, EDTA)
• Sensitivity: <1 pg/ml
• Specificity: Natural and recombinant human CCL8
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: Q24-P99
• Description: For quantitative detection of human CCL8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: CCL8
• Gene Full Name: chemokine (C-C motif) ligand 8
• Alias Symbols: C C motif chemokine 8, CCL 8, Ccl8, CCL8_HUMAN, Chemokine (C C motif) ligand 8, Chemokine ligand 8, HC14, MCP 2, MCP-2, MCP-2(6-76), Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, SCYA10, SCYA8, Small inducible cytokine A8, Small inducible cytokine subfamily A (Cys Cys) member 8, Small-inducible cytokine A8
• NCBI Gene Id: 6355
• Protein Name: C-C motif chemokine 8
• Description of Target: Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.
• Uniprot ID: P80075
• Protein Accession #: NP_005614.2
• Nucleotide Accession #: NM_005623.2