Catalog No: OKEH07279
Size:96T
Price: $875.00
SKU
OKEH07279
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityCanis lupus, Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology CCL8 ELISA Kit (Dog) (OKEH07279) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CCL8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CCL8 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=2.8% (n = 20)
Mean Inter-assay CV%: <=10.0% (n = 20)
ELISA Kit Component
ComponentAmount
CCL8 Microplate96 Wells (12 x 8 Well strips)
CCL8 Lyophilized Standard2
100X Biotinylated CCL8 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Chemotactic factor that attracts monocytes. This protein can bind heparin.
::Subcellular Location: Secreted
Reconstitution and StorageStore as indicated in product manual.
Sensitivity39pg/mL
SpecificityNatural and recombinant Dog C-C motif chemokine 8
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCCL8
Gene Full NameC-C motif chemokine ligand 8
Alias SymbolsC-C motif chemokine 8;chemokine (C-C motif) ligand 8;MCP-2;monocyte chemoattractant protein 2;Monocyte chemotactic protein 2;monocyte chemotactic protein-2;small chemokine ligand 8;small-inducible cytokine A8.
NCBI Gene Id448792
Protein NameQ68AY9
Description of TargetC-C motif chemokine 8
Uniprot IDhttps://www.uniprot.org/uniprot/Q68AY9
Protein Accession #NP_001005255.1
Nucleotide Accession #NM_001005255.1
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