CCL8 ELISA Kit (Bovine) (OKCD02719)

Catalog No: OKCD02719

Size:96WELLS

Bovine CCL8 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus|Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-99%	93-101%	94-104%	80-101%
  EDTA Plasma (n=5)	98-105%	98-105%	91-99%	95-104%
  Heparin Plasma (n=5)	96-104%	92-105%	84-95%	86-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Monocyte Chemotactic Protein 2 (MCP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Monocyte Chemotactic Protein 2 (MCP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Monocyte Chemotactic Protein 2 (MCP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Monocyte Chemotactic Protein 2 (MCP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.62-1000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	79-90	86
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma(n=5)	81-102	97

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monocyte Chemotactic Protein 2 (MCP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monocyte Chemotactic Protein 2 (MCP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CCL8 Microplate: 96 Wells (12 x 8 Well strips)
  • CCL8 Lyophilized Standard: 2
  • 100X Biotinylated CCL8 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture cell supernatant and other biological fluids
• Sensitivity: 6.4 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Monocyte Chemotactic Protein 2 (MCP2). No significant cross-reactivity or interference between Monocyte Chemotactic Protein 2 (MCP2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CCL8
• Gene Full Name: chemokine (C-C motif) ligand 8
• Alias Symbols: C-C motif chemokine 8;C-C motif chemokine 8-like;MCP-2;monocyte chemoattractant protein 2;monocyte chemotactic protein 2;SCYA8;small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2);small-inducible cytokine A8.
• NCBI Gene Id: 281044
• Protein Name: C-C motif chemokine 8
• Description of Target: Chemotactic factor that attracts monocytes. This protein can bind heparin.
• Uniprot ID: Q09141
• Protein Accession #: NP_776432.1
• Nucleotide Accession #: NM_174007.1
• Protein Size (# AA): 99