CCL5 ELISA Kit (Guinea Pig) (OKCD00217)

Catalog No: OKCD00217 (Formerly GWB-KBBUI9)

Size:96WELLS

Guinea Pig CCL5 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Cavia porcellus|Guinea Pig
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	96-104%	95-103%	94-105%	98-105%
  EDTA plasma(n=5)	97-105%	83-102%	89-96%	78-99%
  heparin plasma(n=5)	79-91%	91-99%	85-93%	95-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	86-101	94
  EDTA plasma(n=5)	79-92	85
  heparin plasma(n=5)	96-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CCL5 Microplate: 96 Wells (12 x 8 Well Strips)
  • CCL5 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CCL5 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
• Sensitivity: < 0.055 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). No significant cross-reactivity or interference between Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: Ccl5
• Gene Full Name: C-C motif chemokine ligand 5
• Alias Symbols: C-C motif chemokine 5;RANTES;SCYA5;SIS-delta;small-inducible cytokine A5;T-cell-specific protein RANTES.
• NCBI Gene Id: 100135495
• Protein Name: C-C motif chemokine 5
• Description of Target: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
• Uniprot ID: P97272
• Protein Accession #: NP_001166398
• Nucleotide Accession #: NM_001172927.1
• Protein Size (# AA): 91