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Catalog No: OKCD03899
Size:96WELLS
Price: $850.00
SKU
OKCD03899
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CCL5 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03899)

Datasheets/ManualsPrintable datasheet for CCL5 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03899)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)92-101%79-101%95-103%80-90%
EDTA Plasma (n=5)78-92%89-97%92-105%81-99%
Heparin Plasma (n=5)84-98%87-99%91-104%98-105%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) level in the sample or standard.
ELISA Kit Range13.7-10,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)82-9892
EDTA Plasma (n=5)98-105102
Heparin Plasma (n=5)83-10190
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Ccl5 Microplate96 Wells (12 x 8 Well strips)
Ccl5 Lyophilized Standard2
100X Ccl5 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity13.7 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCcl5
Gene Full Namechemokine (C-C motif) ligand 5
Alias SymbolsC-C motif chemokine 5;MuRa;MuRantes;RA;RANTES;S;Scy;Scya5;SISd;SIS-delta;small-inducible cytokine A5;T-cell-specific protein RANTES;TCP22;TCP228.
NCBI Gene Id20304
Protein NameC-C motif chemokine 5
Description of TargetChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Uniprot IDP30882
Protein Accession #NP_038681
Nucleotide Accession #NM_013653
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